Kuhikar , R and Khan , N and Philip , J and Melinkeri , S and Kale , V and Limaye , L (2020) Transforminggrowth factor β1 accelerates and enhances in vitro red blood cell formation from hematopoietic stem cells by stimulating mitophagy. Stem Cell Research and Therapy, 11 (1). p. 71.

Text 29. Dr. Limaye LS. (Stem Cell Res & Therapy) open access.pdf Restricted to Repository staff only Download (1761Kb) | Request a copy

Abstract

Generation of red blood cells (RBCs) from hematopoietic stem cells (HSCs) in vitro takes about 21 days, making it unaffordable for clinical applications. Acceleration of the in vitro erythropoiesis process by using small molecules could eventually make the large-scale production of these cells commercially viable. Transforming Growth Factor β1 (TGF-β1) has been shown to have a dose-dependent activity on the HSCs: at high concentration it inhibits, whereas at low concentration it stimulates the HSCs growth. At high concentration, it also inhibits erythropoiesis but accelerates terminal erythroid differentiation of cell lines and erythroid progenitors. Here we examined whether the use of low concentration of TGF-β1 would be beneficial for increasing RBC production by stimulating HSC growth and also supporting erythroid differentiation. Such a strategy could make RBC production in vitro more efficient and cost-effective for clinical applications.

Item Type:	Article
Subjects:	Stem Cell Biology
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	04 Aug 2020 16:12
Last Modified:	08 Dec 2021 10:58
URI:	http://nccs.sciencecentral.in/id/eprint/804

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