Joglekar, MV and Satoor, SN and Wong, WKM and Cheng, F and Ma RCW, RCW and Hardikar, AA (2020) An Optimised Step-by-Step Protocol for Measuring Relative Telomere Length. Methods and Protocols, 3 (2). p. 27.

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Abstract

Telomeres represent the nucleotide repeat sequences at the ends of chromosomes and are essential for chromosome stability. They can shorten at each round of DNA replication mainly because of incomplete DNA synthesis of the lagging strand. Reduced relative telomere length is associated with aging and a range of disease states. Different methods such as terminal restriction fragment analysis, real-time quantitative PCR (qPCR) and fluorescence in situ hybridization are available to measure telomere length; however, the qPCR-based method is commonly used for large population-based studies. There are multiple variations across qPCR-based methods, including the choice of the single-copy gene, primer sequences, reagents, and data analysis methods in the different reported studies so far. Here, we provide a detailed step-by-step protocol that we have optimized and successfully tested in the hands of other users. This protocol will help researchers interested in measuring relative telomere lengths in cells or across larger clinical cohort/study samples to determine associations of telomere length with health and disease.

Item Type:	Article
Subjects:	Cell Biology
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	19 Jan 2021 06:15
Last Modified:	29 Nov 2021 11:10
URI:	http://nccs.sciencecentral.in/id/eprint/856

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