Prakash , O. and Munot , H. and Nimonkar, Y. and Sharma, M. and Kumbhare, S. and Shouche , Y.S. (2014) Description of Pelistega indica sp. nov., isolated from human gut. International Journal of Systematic and Evolutionary Microbiology, 64. pp. 1389-1394.

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Abstract

A Gram-stain-negative, motile, non-spore-forming, coccoid bacterium was isolated from a stool sample of a healthy human subject and formed cream colour colonies on tryptic soy agar. Almost full-length (1500 bp) small subunit rRNA (16S rRNA) gene sequences were generated and a similarity search was conducted by BLAST. The results of the similarity search indicated that the bacterium belongs to the class Betaproteobacteria, family Alcaligenaceae. It showed maximum sequence similarity (96.5 %) with Pelistega europaea CCUG 39967T followed by Advenella mimigardefordensis DSM 17166T (96.1 %) and Taylorella asinigenitalis LMG 19572T (95.3 %). The DNA G+C content of strain HM-7T was 42 mol%. Strain HM-7T contained C14 : 0, C16 : 0, C16 : 0 3-OH and C18 : 0 as the dominant fatty acids. Morphological, physiological and biochemical data also indicated that strain HM-7T represents a member of the genus Pelistega, but at the same time distinguished it from Pelistega europaea CCUG 39967T, the only species of the genus with a validly published name. Based on polyphasic characterization we conclude that the bacterium represents a novel species of the genus Pelistega and propose the name Pelistega indica sp. nov., with strain HM-7T (5MCC 2185T5DSM 27484T) as the type strain of the species.

Item Type:	Article
Subjects:	Insect Molecular Biology
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	24 Apr 2015 07:08
Last Modified:	01 Jul 2015 09:17
URI:	http://nccs.sciencecentral.in/id/eprint/119

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