Dastager , S.G. and Mawlankar, R. and Mual , P. and Verma, A. and Krishnamurthi , S. and Joseph , N. and Shouche, Y.S. (2015) Bacillus encimensis sp. nov. isolated from marine sediment. Int J Syst Evol Microbiol. , 65 (5). pp. 1421-1425.

Full text not available from this repository. (Request a copy)

Abstract

A Gram-stain-positive, rod-shaped, endospore-forming, aerobic bacterium designated SGD-V-25(T) was isolated from Veraval sediment sample, India. Strain SGD-V-25(T) was capable of growing at 25-50 °C (optimum 37 °C), pH 6-12 (optimum pH 7.0) and with 0-5% (w/v) NaCl. The taxonomic position of this strain was deduced using a polyphasic approach and the 16S rRNA gene sequence analysis showed that the isolate belongs to the phylum Firmicutes , forming the cluster with Bacillus badius MTCC 1548(T), with which it shares highest similarity of 99.1% with 13 nt differences. Other type strains of the genus Bacillus showed less than 96% similarity. The cell wall contained meso-diaminopimelic acid as the diagnostic diamino acid. The polar lipid profile of strain SGD-V-25(T) showed the presence of diphosphatidylglycerol, phosphatidylglycerol, phsophoglycolipid and two aminophospholipids. The predominant isoprenoid quinone was MK-7. The major cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0, anteiso-C17 : 0, iso-C16 : 0, C16 : 1ω11c and C16 : 0. The genomic DNA G+C content of strain SGD-V-25(T) was 37.6 mol%. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA hybridization, strain SGD-V-25(T) could be clearly distinguished from closely related members of the genus Bacillus , and the name Bacillus encimensis sp. nov., is proposed to accommodate this strain. The type strain is SGD-V-25(T) ( =NCIM 5513(T) =DSM 28241(T)).

Item Type:	Article
Subjects:	Insect Molecular Biology
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	21 Dec 2016 07:36
Last Modified:	21 Dec 2016 07:36
URI:	http://nccs.sciencecentral.in/id/eprint/353

Actions (login required)

View Item