Shaligram, S. and Kumbhare, S.V. and Dhotre, D.P. and Muddeshwar , M.G. and Kapley, A. and Joseph, N. and Purohit , H.P. and Shouche, Y.S. and Pawar, S.P. (2016) Genomic and functional features of the biosurfactant producing Bacillus sp. AM13. Funct Integr Genomics, 16 (5). pp. 557-566. ISSN 2016 Sep;16(5):557-66

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Abstract

Genomic studies provide deeper insights into secondary metabolites produced by diverse bacterial communities, residing in various environmental niches. This study aims to understand the potential of a biosurfactant producing Bacillus sp. AM13, isolated from soil. An integrated approach of genomic and chemical analysis was employed to characterize the antibacterial lipopeptide produced by the strain AM13. Genome analysis revealed that strain AM13 harbors a nonribosomal peptide synthetase (NRPS) cluster; highly similar with known biosynthetic gene clusters from surfactin family: lichenysin (85 %) and surfactin (78 %). These findings were substantiated with supplementary experiments of oil displacement assay and surface tension measurements, confirming the biosurfactant production. Further investigation using LCMS approach exhibited similarity of the biomolecule with biosurfactants of the surfactin family. Our consolidated effort of functional genomics provided chemical as well as genetic leads for understanding the biochemical characteristics of the bioactive compound.

Item Type:	Article
Subjects:	Insect Molecular Biology
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	27 Dec 2016 09:26
Last Modified:	27 Dec 2016 09:26
URI:	http://nccs.sciencecentral.in/id/eprint/377

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