Banskar, S. and Bhute, S.S. and Suryavanshi , M.V. and Punekar, S. and Shouche, Y.S. (2016) Microbiome analysis reveals the abundance of bacterial pathogens in Rousettus leschenaultii guano. Scientific Report, 15 (6). p. 36948.

Text 8.Dr. Shouche Y.S. (Sci. Report) open access.pdf Download (1811Kb)

Abstract

Bats are crucial for proper functioning of an ecosystem. They provide various important services to ecosystem and environment. While, bats are well-known carrier of pathogenic viruses, their possible role as a potential carrier of pathogenic bacteria is under-explored. Here, using culture-based approach, employing multiple bacteriological media, over thousand bacteria were cultivated and identified from Rousettus leschenaultii (a frugivorous bat species), the majority of which were from the family Enterobacteriaceae and putative pathogens. Next, pathogenic potential of most frequently cultivated component of microbiome i.e. Escherichia coli was assessed to identify its known pathotypes which revealed the presence of virulent factors in many cultivated E. coli isolates. Applying in-depth bacterial community analysis using high-throughput 16 S rRNA gene sequencing, a high inter-individual variation was observed among the studied guano samples. Interestingly, a higher diversity of bacterial communities was observed in decaying guano representative. The search against human pathogenic bacteria database at 97% identity, a small proportion of sequences were found associated to well-known human pathogens. The present study thus indicates that this bat species may carry potential bacterial pathogens and advice to study the effect of these pathogens on bats itself and the probable mode of transmission to humans and other animals.

Item Type:	Article
Additional Information:	This is Open Access Article
Subjects:	Insect Molecular Biology
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	28 Dec 2016 07:14
Last Modified:	02 Mar 2021 11:15
URI:	http://nccs.sciencecentral.in/id/eprint/390

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