Shah , S and Chand , K and Rekadwad , B and Shouche , YS and Sharma , J and Pant , B (2021) A prospectus of plant growth promoting endophytic bacterium from orchid (Vanda cristata). BMC Biotechnolgy, 21 (1). p. 16.

Text 83.-1 Dr. Shouche YS (BMC Biotechnolgy) open access.pdf Restricted to Repository staff only Download (1033Kb) | Request a copy

Abstract

Background A plant growth-promoting endophytic bacterium PVL1 isolated from the leaf of Vanda cristata has the ability to colonize with roots of plants and protect the plant. PVL1 was isolated using laboratory synthetic media. 16S rRNA gene sequencing method has been employed for identification before and after root colonization ability. Results Original isolated and remunerated strain from colonized roots were identified as Bacillus spp. as per EzBiocloud database. The presence of bacteria in the root section of the plantlet was confirmed through Epifluorescence microscopy of colonized roots. The in-vitro plantlet colonized by PVL1 as well as DLMB attained higher growth than the control. PVL1 capable of producing plant beneficial phytohormone under in vitro cultivation. HPLC and GC-MS analysis suggest that colonized plants contain Indole Acetic Acid (IAA). The methanol extract of Bacillus spp., contains 0.015 μg in 1 μl concentration of IAA. PVL1 has the ability to produce antimicrobial compounds such as ethyl iso-allocholate, which exhibits immune restoring property. One-way ANOVA shows that results were statistically significant at P ≤ 0.05 level. Conclusions Hence, it has been concluded that Bacillus spp. PVL1 can promote plant growth through secretion of IAA during root colonization and ethyl iso-allocholate to protect plants from foreign infections. Thus, this study supports to support Koch’s postulates of bacteria establishment.

Item Type:	Article
Subjects:	Insect Molecular Biology
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	26 Apr 2021 11:45
Last Modified:	29 Nov 2021 10:54
URI:	http://nccs.sciencecentral.in/id/eprint/942

Actions (login required)

View Item